Missouri State-Mountain Grove is a 190-acre campus located in Mountain Grove on the Ozarks Plateau in rural south-central Missouri. Provost Frank Einhellig is Chancellor of the campus. Headed by Dean Ron Del Vecchio, the William H. Darr College of Agriculture operates the Missouri State Fruit Experiment Station that includes the Missouri State Winery and Distillery.

The 12 acres east of the highway features Historic Faurot Hall housing the Paul Evans Library of Fruit Science, Shepard Hall, the Classroom Building, the Research Winery, the Fruit Processing Education Building, the Sales Dock, Greenhouses, and the Ozark Arboretum. The 180 acres west of highway 95 features the Shop and Machine Storage Building, the Duplex, the Horse Barn, Pine Pond, the Horticulture Demonstration Area, and research and demonstration plantings.

Graduate students work on their research on the Mountain Grove plantings and in the laboratories. The VESTA program offers a course of study that leads to a Certificate or Associate of Applied Science Degree in Viticulture or Enology and is offered through Missouri State-West Plains. The Missouri State-West Plains extended campus is located just south of the State Fruit Experiment Station in Shannon Hall.